Optimization of the Extraction Technology and Assessment of Antioxidant Activity of Chlorogenic Acid-Rich Extracts From <b><i>Eucommia ulmoides</i></b> Leaves

Chlorogenic acid has been proved to have cardiovascular protection, antibacterial, antiviral, hemostatic, and hypolipidemia effects. Modern scientific research on the bioactivity of chlorogenic extended fields food, medicine, health care daily-use chemical industry. The aim this was optimize extraction conditions for from Eucommia ulmoides (Eucommiaceae) leaves. significant variables were screened optimized by a combination Plackett-Burman test Box-Behnken design. Optimum parameters with ethanol concentration 50%, solvent pH value 3, particle size 60 mesh determined according variance analysis contour plots. Under these conditions, yield up 4.36 mg/g, which basically consistent theoretical prediction 4.50 mg/g. This study also potential antioxidant activity E. optimal extract leaves rich in showed highest FRAP method, 219.8 μM Trolox equivalents (TE) per g weight (EW) (μM TE/g EW). DPPH method gave similar (168 EW) ABTS (152 established process efficient recovery leaves, encouraging its valorization as cheap sustainable alternative isolation acid.